| First Author | Tassone NM | Year | 2019 |
| Journal | Am J Physiol Renal Physiol | Volume | 317 |
| Issue | 6 | Pages | F1503-F1512 |
| PubMed ID | 31532245 | Mgi Jnum | J:293372 |
| Mgi Id | MGI:6452736 | Doi | 10.1152/ajprenal.00160.2019 |
| Citation | Tassone NM, et al. (2019) Stem cell antigen/Ly6a protects against bladder fibrosis in mice. Am J Physiol Renal Physiol 317(6):F1503-F1512 |
| abstractText | We have defined a population of stem cell antigen (Sca)-1(+)/CD34(+)/lin(-) mesenchymal stem cells in the mouse urinary bladder. These cells are reduced after partial bladder outlet obstruction (PO). To test the role of Sca-1 expressed by these cells, we analyzed bladders from Sca-1 knockout (KO) mice in both uninjured male mice and male mice subjected to PO. We found that loss of Sca-1 alone had little effect on bladder development or function but reduced the total number of mesenchymal stem cells by 30%. After PO, bladders from Sca-1-null KO male mice were larger, with more collagen and less muscle, than obstructed wild-type mice. Steady-state levels of caldesmon were significantly reduced and levels of fibroblast-specific protein 1 were significantly increased in Sca-1 KO mice compared with wild-type mice after PO. In investigating the effects of PO on cell proliferation, we found that loss of Sca-1 changed the timing of cell division in CD34(+)/lin(-), collagen-producing, and smooth muscle cells. PO in combination with loss of Sca-1 drastically reduced the ability of CD34(+)/lin(-) cells to form colonies in vitro. Our findings therefore support the hypothesis that Sca-1 protects the bladder from fibrotic remodeling after obstruction, in part by influencing the proliferation of cells responding to the injury. |
