| Primary Identifier | MGI:2450149 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Chrne |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 1.4 kb fragment encompassing exons 2 through 5 was replaced with neomycin selection cassette inserted by homologous recombination. The deleted region encodoed 150 amino terminal residues, which are essential for proper subunit assembly of the acetylcholine receptor. RNAse protection identified reduced levels of transcript in homozygous mutant muscle tissue. Immunostaining using an antibody directed against the carboxy terminal indicated an absence of protein at neuromuscular junctions. |
