| First Author | Wang W | Year | 2022 |
| Journal | Nat Immunol | Volume | 23 |
| Issue | 7 | Pages | 1052-1062 |
| PubMed ID | 35726060 | Mgi Jnum | J:350412 |
| Mgi Id | MGI:7662425 | Doi | 10.1038/s41590-022-01232-z |
| Citation | Wang W, et al. (2022) TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors. Nat Immunol 23(7):1052-1062 |
| abstractText | The high mobility group (HMG) transcription factor TCF-1 is essential for early T cell development. Although in vitro biochemical assays suggest that HMG proteins can serve as architectural elements in the assembly of higher-order nuclear organization, the contribution of TCF-1 on the control of three-dimensional (3D) genome structures during T cell development remains unknown. Here, we investigated the role of TCF-1 in 3D genome reconfiguration. Using gain- and loss-of-function experiments, we discovered that the co-occupancy of TCF-1 and the architectural protein CTCF altered the structure of topologically associating domains in T cell progenitors, leading to interactions between previously insulated regulatory elements and target genes at late stages of T cell development. The TCF-1-dependent gain in long-range interactions was linked to deposition of active enhancer mark H3K27ac and recruitment of the cohesin-loading factor NIPBL at active enhancers. These data indicate that TCF-1 has a role in controlling global genome organization during T cell development. |
