| First Author | Xiang X | Year | 2022 |
| Journal | FASEB J | Volume | 36 |
| Issue | 6 | Pages | e22335 |
| PubMed ID | 35506565 | Mgi Jnum | J:344962 |
| Mgi Id | MGI:7345737 | Doi | 10.1096/fj.202101518R |
| Citation | Xiang X, et al. (2022) Impaired reciprocal regulation between SIRT6 and TGF-beta signaling in fatty liver. FASEB J 36(6):e22335 |
| abstractText | Dysregulated transforming growth factor-beta (TGF-beta) signaling contributes to fibrotic liver disease and hepatocellular cancer (HCC), both of which are associated with fatty liver disease. SIRT6 limits fibrosis by inhibiting TGF-beta signaling through deacetylating SMAD2 and SMAD3 and limits lipogenesis by inhibiting SREBP1 and SREBP2 activity. Here, we showed that, compared to wild-type mice, high-fat diet-induced fatty liver is worse in TGF-beta signaling-deficient mice (SPTBN1(+/-) ) and the mutant mice had reduced SIRT6 abundance in the liver. Therefore, we hypothesized that altered reciprocal regulation between TGF-beta signaling and SIRT6 contributes to these liver pathologies. We found that deficiency in SMAD3 or SPTBN1 reduced SIRT6 mRNA and protein abundance and impaired TGF-beta induction of SIRT6 transcripts, and that SMAD3 bound to the SIRT6 promoter, suggesting that an SMAD3-SPTBN1 pathway mediated the induction of SIRT6 in response to TGF-beta. Overexpression of SIRT6 in HCC cells reduced the expression of TGF-beta-induced genes, consistent with the suppressive role of SIRT6 on TGF-beta signaling. Manipulation of SIRT6 abundance in HCC cells altered sterol regulatory element-binding protein (SREBP) activity and overexpression of SIRT6 reduced the amount of acetylated SPTBN1 and the abundance of both SMAD3 and SPTBN1. Furthermore, induction of SREBP target genes in response to SIRT6 overexpression was impaired in SPTBN1 heterozygous cells. Thus, we identified a regulatory loop between SIRT6 and SPTBN1 that represents a potential mechanism for susceptibility to fatty liver in the presence of dysfunctional TGF-beta signaling. |
