| First Author | Becker HM | Year | 2013 |
| Journal | J Immunol | Volume | 190 |
| Issue | 8 | Pages | 4305-14 |
| PubMed ID | 23509351 | Mgi Jnum | J:195298 |
| Mgi Id | MGI:5477868 | Doi | 10.4049/jimmunol.1202097 |
| Citation | Becker HM, et al. (2013) alpha1beta1 Integrin-Mediated Adhesion Inhibits Macrophage Exit from a Peripheral Inflammatory Lesion. J Immunol 190(8):4305-14 |
| abstractText | Integrins are adhesion molecules critical for the recruitment of leukocytes from blood into peripheral tissues. However, whether integrins are also involved in leukocyte exit from peripheral tissues via afferent lymphatics to the draining lymph node remains poorly understood. In this article, we show that adhesion by the collagen IV-binding integrin alpha1beta1 unexpectedly inhibited macrophage exit from inflamed skin. We monitored macrophages exiting mouse footpads using a newly developed in situ pulse labeling technique. Blockade of alpha1beta1 integrin or genetic deletion (Itga1(-/-)) increased macrophage exit efficiency. Chemotaxis assays through collagen IV showed more efficient migration of Itga1(-/-) macrophages relative to wild type. Given that macrophages are key orchestrators of inflammation, alpha1beta1 integrin adhesion may represent a mechanism for regulating inflammatory responses by controlling macrophage exit or persistence in inflamed tissues. |
