| Primary Identifier | MGI:2653986 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Gria1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Alanine amino acid substitutions were introduced to phosphorylation sites serine 831 (S831A) and serine 845 (S845A) in exon 17 of the gene. A floxed neomycin resistance cassette was inserted upstream of exon 17 for positive selection. Mutant animals were crossed to cre deleter strain Tg(CMV-cre)1Nagy to removed the floxed neo cassette, leaving behind the 2 amino acid substitutions. The targeted mutation was confirmed using immunoblot with phosphorylation site-specific antibodies. |
