| Primary Identifier | MGI:2652788 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Krt10 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An arginine to cysteine mutation was introduced to codon 154 (R154C) of exon 1 along with a floxed neo cassette via homologous recombination. R154C is a mutation commonly found in human epidermolytic hyperkeratosis (EHK). The floxed neo cassette prevents expression of the mutant allele until its removal by cre-mediated recombination. RNA analysis revealed the mutant allele is expressed at 35%-40% of wild-type. |
