| First Author | Amin FZ | Year | 2018 |
| Journal | BMC Res Notes | Volume | 11 |
| Issue | 1 | Pages | 449 |
| PubMed ID | 29986746 | Mgi Jnum | J:263769 |
| Mgi Id | MGI:6191801 | Doi | 10.1186/s13104-018-3563-7 |
| Citation | Amin FZ, et al. (2018) Deterioration of alveolar development in mice with both HIF-3alpha knockout and HIF-2alpha knockdown. BMC Res Notes 11(1):449 |
| abstractText | OBJECTIVE: Earlier studies from our group using hypoxia-inducible factor 3alpha knockout mice showed impairments in lung remodeling and lung endothelial cells. Another research from our group demonstrated that impaired expression of hypoxia-inducible factor 2alpha induced compensatory expression of hypoxia-inducible factor 1alpha in hypoxia-inducible factor 2alpha knockdown mice. The present study uncovers more insights by extending the investigation, utilizing mice with both hypoxia-inducible factor 3alpha knockout and hypoxia-inducible factor 2alpha knockdown. RESULTS: No mice with both hypoxia-inducible factor 3alpha knockout and hypoxia-inducible factor 2alpha knockdown died immediately after birth. The mice with both hypoxia-inducible factor 3alpha knockout and hypoxia-inducible factor 2alpha knockdown exhibited impaired alveolar sacs and lung alveolar structure and decreased endothelial cell numbers. Analysis of relative mRNA expression revealed depressed expressions of hypoxia-inducible factor 1alpha, vascular cell adhesion molecule 1, vascular endothelial cadherin, angiopoietin 2, Tie-2, and vascular endothelial growth factor in the lungs of mice with both hypoxia-inducible factor 3alpha knockout and hypoxia-inducible factor 2alpha knockdown compared to that in wild-type mice. Further analysis is needed to elucidate the impaired development occurred in the lung endothelial cells. |
