| First Author | Ge CX | Year | 2017 |
| Journal | Biochem Biophys Res Commun | Volume | 493 |
| Issue | 4 | Pages | 1402-1409 |
| PubMed ID | 28965953 | Mgi Jnum | J:305773 |
| Mgi Id | MGI:6710905 | Doi | 10.1016/j.bbrc.2017.09.152 |
| Citation | Ge CX, et al. (2017) iRhom2 deficiency relieves TNF-alpha associated hepatic dyslipidemia in long-term PM2.5-exposed mice. Biochem Biophys Res Commun 493(4):1402-1409 |
| abstractText | Accumulating researches reported that particulate matter (PM2.5) is a risk factor for developing various diseases, including metabolic syndrome. Recently, inactive rhomboid protein 2 (iRhom2) was considered as a necessary modulator for shedding of tumor necrosis factor-alpha (TNF-alpha) in immune cells. TNF-alpha, a major pro-inflammatory cytokine, was linked to various pathogenesis of diseases, including dyslipidemia. Here, wild type (WT) and iRhom2-knockout (iRhom2(-/-)) mice were used to investigate the effects of iRhom2 on PM2.5-induced hepatic dyslipidemia. The hepatic histology, inflammatory response, glucose tolerance, serum parameters and gene expressions were analyzed. We found that long-term inhalation of PM2.5 resulted in hepatic steatosis. And a significant up-regulation of iRhom2 in liver tissues was observed, accompanied with elevated TNF-alpha, TNF-alpha converting enzyme (TACE), TNFalpha receptor (TNFR)2 and various inflammatory cytokines expressions. Additionally, PM2.5 treatment caused TG and TC accumulation in serum and liver, probably attributed to changes of genes modulating lipid metabolism. Intriguingly, hepatic injury and dyslipidemia were attenuated by iRhom2(-/-) in mice with PM2.5 challenge. In vitro, iRhom2-knockdwon reduced TNF-alpha expressions and its associated inflammatory cytokines in Kupffer cells, implying that liver-resident macrophages played an important role in regulating hepatic inflammation and lipid metabolism in cells treated with PM2.5. The findings indicated that long-term PM2.5 exposure caused hepatic steatosis and dyslipidemia through triggering inflammation, which was, at least partly, dependent on iRhom2/TNF-alpha pathway in liver-resident macrophages. |
