First Author | Noro T | Year | 2015 |
Journal | Invest Ophthalmol Vis Sci | Volume | 56 |
Issue | 8 | Pages | 5012-9 |
PubMed ID | 26230766 | Mgi Jnum | J:230946 |
Mgi Id | MGI:5766577 | Doi | 10.1167/iovs.15-17142 |
Citation | Noro T, et al. (2015) Spermidine Ameliorates Neurodegeneration in a Mouse Model of Normal Tension Glaucoma. Invest Ophthalmol Vis Sci 56(8):5012-9 |
abstractText | PURPOSE: To assess the therapeutic potential of spermidine in mice with excitatory amino acid carrier 1 (EAAC1) deletion (EAAC1 knockout [KO] mice), a mouse model of normal tension glaucoma. METHODS: Spermidine, at 30 mM in drinking water, was administered to EAAC1 KO mice from 5 to 12 weeks old. Optical coherence tomography, multifocal electroretinograms, and the measurement of intraocular pressure (IOP) were performed at 5, 8, and 12 weeks old. Histopathology analyses were carried out at 8 and 12 weeks old, and immunoblot and immunohistochemical analyses of 4-hydroxy-2-nonenal (4-HNE) in the retina were performed at 8 weeks old. RESULTS: Spermidine ameliorated retinal degeneration and improved visual function in EAAC1 KO mice at both 8 and 12 weeks old, without affecting IOP. A significant increase of 4-HNE was observed in vehicle-treated EAAC1 KO mice, but spermidine treatment reduced this increase, suggesting that spermidine alleviated the severity of the glaucoma-like phenotype by acting as an antioxidant. CONCLUSIONS: The results from this study suggest that oral spermidine administration could be a useful treatment for retinal degenerative disorders including glaucoma. |