| First Author | Aloway JA | Year | 2024 |
| Journal | Differentiation | Volume | 138 |
| Pages | 100791 | PubMed ID | 38941819 |
| Mgi Jnum | J:352882 | Mgi Id | MGI:7706320 |
| Doi | 10.1016/j.diff.2024.100791 | Citation | Aloway JA, et al. (2024) Generation of a Wt1 conditional deletion, nuclear red fluorescent protein reporter allele in the mouse. Differentiation 138:100791 |
| abstractText | A Wt1 conditional deletion, nuclear red fluorescent protein (RFP) reporter allele was generated in the mouse by gene targeting in embryonic stem cells. Upon Cre-mediated recombination, a deletion allele is generated that expresses RFP in a Wt1-specific pattern. RFP expression was detected in embryonic and adult tissues known to express Wt1, including the kidney, mesonephros, and testis. In addition, RFP expression and WT1 co-localization was detected in the adult uterine stroma and myometrium, suggesting a role in uterine function. Crosses with Wnt7a-Cre transgenic mice that express Cre in the Mullerian duct epithelium activate Wt1-directed RFP expression in the epithelium of the oviduct but not the stroma and myometrium of the uterus. This new mouse strain should be a useful resource for studies of Wt1 function and marking Wt1-expressing cells. |
