| First Author | Simon CS | Year | 2018 |
| Journal | Biol Open | Volume | 7 |
| Issue | 12 | PubMed ID | 30530745 |
| Mgi Jnum | J:268273 | Mgi Id | MGI:6269268 |
| Doi | 10.1242/bio.036517 | Citation | Simon CS, et al. (2018) A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open 7(12):bio036517 |
| abstractText | The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and in adult organs. These include the primitive endoderm of the blastocyst, visceral endoderm of the early post-implantation embryo, as well as lateral plate mesoderm, developing heart, liver, lung and gonads. Here, we generate a novel Gata4 targeted allele used to generate both a Gata4(H2B-GFP) transcriptional reporter and a Gata4(FLAG) fusion protein to analyse dynamic expression domains. We demonstrate that the Gata4(H2B-GFP) transcriptional reporter faithfully recapitulates known sites of Gata4 mRNA expression and correlates with endogenous GATA4 protein levels. This reporter labels nuclei of Gata4 expressing cells and is suitable for time-lapse imaging and single cell analyses. As such, this Gata4(H2B-GFP) allele will be a useful tool for studying Gata4 expression and transcriptional regulation.This article has an associated First Person interview with the first author of the paper. |
