| First Author | Shioda N | Year | 2017 |
| Journal | Mol Psychiatry | Volume | 22 |
| Issue | 8 | Pages | 1205-1222 |
| PubMed ID | 27922607 | Mgi Jnum | J:305138 |
| Mgi Id | MGI:6511357 | Doi | 10.1038/mp.2016.200 |
| Citation | Shioda N, et al. (2017) Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRbeta signaling in striatopallidal medium spiny neurons. Mol Psychiatry 22(8):1205-1222 |
| abstractText | Aberrant dopamine D2 receptor (D2R) activity is associated with neuropsychiatric disorders, making those receptors targets for antipsychotic drugs. Here, we report that novel signaling through the intracellularly localized D2R long isoform (D2LR) elicits extracellular signal-regulated kinase (ERK) activation and dendritic spine formation through Rabex-5/platelet-derived growth factor receptor-beta (PDGFRbeta)-mediated endocytosis in mouse striatum. We found that D2LR directly binds to and activates Rabex-5, promoting early-endosome formation. Endosomes containing D2LR and PDGFRbeta are then transported to the Golgi apparatus, where those complexes trigger Galphai3-mediated ERK signaling. Loss of intracellular D2LR-mediated ERK activation decreased neuronal activity and dendritic spine density in striatopallidal medium spiny neurons (MSNs). In addition, dendritic spine density in striatopallidal MSNs significantly increased following treatment of striatal slices from wild-type mice with quinpirole, a D2R agonist, but those changes were lacking in D2LR knockout mice. Moreover, intracellular D2LR signaling mediated effects of a typical antipsychotic drug, haloperidol, in inducing catalepsy behavior. Taken together, intracellular D2LR signaling through Rabex-5/PDGFRbeta is critical for ERK activation, dendritic spine formation and neuronal activity in striatopallidal MSNs of mice. |
