| Primary Identifier | MGI:2661167 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rtn4 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The first of 6 exons common to isoforms A, B, and C was replaced with a floxed neo cassette inserted by homologous recombination. The deleted exon encodes the first transmembrane domain and a portion of an extracellular loop. While a shortened C transcript was identified by Northern blot analysis, A and B transcripts were undetected in homozygous mutant mice. RT-PCR and sequence analysis showed the aberrant transcript to lack the neo cassette (as well as the 66 codons it had replaced) and to contain a frameshift mutation after the first 11 codons. |
