| First Author | Shehat MG | Year | 2019 |
| Journal | J Biol Chem | Volume | 294 |
| Issue | 26 | Pages | 10365-10378 |
| PubMed ID | 31113864 | Mgi Jnum | J:281034 |
| Mgi Id | MGI:6368732 | Doi | 10.1074/jbc.RA118.007218 |
| Citation | Shehat MG, et al. (2019) RIP2 promotes FcgammaR-mediated reactive oxygen species production. J Biol Chem 294(26):10365-10378 |
| abstractText | Receptor-interacting protein 2 (RIP2) is a kinase that mediates signaling downstream of the bacterial peptidoglycan sensors NOD1 and NOD2. Genetic loss or pharmaceutical inhibition of RIP2 has been shown to be beneficial in multiple inflammatory disease models with the effects largely attributed to reducing proinflammatory signaling downstream of peptidoglycan recognition. However, given the widespread expression of this kinase and its reported interactions with numerous other proteins, it is possible that RIP2 may also function in roles outside of peptidoglycan sensing. In this work, we show that RIP2 undergoes tyrosine phosphorylation and activation in response to engagement of the Fc gamma receptor (FcgammaR). Using bone marrow-derived macrophages from WT and RIP2-KO mice, we show that loss of RIP2 leads to deficient FcgammaR signaling and reactive oxygen species (ROS) production upon FcgammaR cross-linking without affecting cytokine secretion, phagocytosis, or nitrate/nitrite production. The FcgammaR-induced ROS response was still dependent on NOD2, as macrophages deficient in this receptor showed similar defects. Mechanistically, we found that different members of the Src family kinases (SFKs) can promote RIP2 tyrosine phosphorylation and activation. Altogether, our findings suggest that RIP2 is functionally important in pathways outside of bacterial peptidoglycan sensing and that involvement in such pathways may depend on the actions of SFKs. These findings will have important implications for future therapies designed to target this kinase. |
