| First Author | Tober J | Year | 2008 |
| Journal | Blood | Volume | 111 |
| Issue | 5 | Pages | 2636-9 |
| PubMed ID | 18174377 | Mgi Jnum | J:131538 |
| Mgi Id | MGI:3773941 | Doi | 10.1182/blood-2007-11-124685 |
| Citation | Tober J, et al. (2008) Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb. Blood 111(5):2636-9 |
| abstractText | Hematopoiesis initiates within the yolk sac of mammalian embryos in overlapping primitive and definitive waves, each containing erythroid and megakaryocyte progenitors. c-myb-null mouse fetuses lack definitive erythrocytes but contain primitive erythroblasts and hepatic megakaryocytes. However, it is unclear if c-myb-null embryos harbor definitive erythroid or any megakaryocyte progenitors. We determined that c-myb was not expressed in primitive erythroid precursors and that c-myb-null embryos had normal primitive erythroid and megakaryocyte progenitor numbers and kinetics between embryonic day (E) 7.0 and E9.0. While primitive hematopoiesis is c-myb-independent, no definitive erythroid potential was detected in c-myb-null embryos, confirming that definitive erythropoiesis, beginning at E8.25 in the yolk sac, is completely c-myb-dependent. In contrast, reduced numbers of megakaryocyte progenitors with restricted proliferative capacity persist in E10.5 yolk sac and E11.5 liver. Despite this impaired megakaryocyte potential, c-myb-null fetuses had normal platelet numbers at E12.5 but became thrombocytopenic by E15.5, suggesting that c-myb is required for sustained thrombopoiesis. |
