| First Author | Vinay DS | Year | 2003 |
| Journal | Immunol Cell Biol | Volume | 81 |
| Issue | 3 | Pages | 176-84 |
| PubMed ID | 12752681 | Mgi Jnum | J:83722 |
| Mgi Id | MGI:2663358 | Doi | 10.1046/j.1440-1711.2003.01153.x |
| Citation | Vinay DS, et al. (2003) Immunity in the absence of CD28 and CD137 (4-1BB) molecules. Immunol Cell Biol 81(3):176-84 |
| abstractText | We report the generation and immune regulation of mice that are deficient in CD28 and 4-1BB (CD137) genes. These mice were viable, fertile and did not display any overt abnormalities and had a normal T cell phenotype in thymus and spleen. Proliferative responses to anti-CD3 and ConA were enhanced in 4-1BB-/- but not in either CD28-/- or double mutant mice, while levels of interleukin-2 were decreased in all mutant mice. Although the 4-1BB-/- mice displayed increased basal levels of most immunoglobulin isotypes tested, the plateau levels of immunoglobulin G2a, immunoglobulin G2b and immunoglobulin A were particularly high compared to wild type controls. The immunoglobulin class switch to T-dependent antigen was normal in 4-1BB-/- mice but was greatly affected in both CD28-/- and 4-1BB-/- CD28-/- mice. Vesicular stomatitis virus-specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability was differentially reduced in all mutant mice. Contact sensitivity to allergens showed marginal but not significant change in ear thickness in 4-1BB-/- mice, but an ability to mount contact hypersensitivity to the same antigens was greatly curtailed in CD28-/- and double mutant mice. |
