| Primary Identifier | MGI:2667231 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rasgrf1 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 5 and 6 in the catalytic domain were replaced with a PGKneomycin cassette. This inactivates all potential splicing isoforms. Proper insertion and absence of expression were demonstrated by Sothern blot analysis and RT-PCR. Absence of protein was confirmed by immunoblotting. |
