| First Author | Ohayon A | Year | 2010 |
| Journal | Infect Immun | Volume | 78 |
| Issue | 10 | Pages | 4195-205 |
| PubMed ID | 20660606 | Mgi Jnum | J:164251 |
| Mgi Id | MGI:4830948 | Doi | 10.1128/IAI.00465-10 |
| Citation | Ohayon A, et al. (2010) Protein Kinase C {theta} Deficiency Increases Resistance of C57BL/6J Mice to Plasmodium berghei Infection-Induced Cerebral Malaria. Infect Immun 78(10):4195-205 |
| abstractText | Protein kinase C theta (PKCtheta) functions as a core component of the immunological synapse and serves as a key protein in the integrated T-cell antigen receptor (TCR)/CD28-induced signaling cascade leading to T-cell activation. However, the involvement of PKCtheta in host-mediated immune responses to pathogens has not been thoroughly investigated. We tested the consequences of PKCtheta ablation on the host response to infection by Plasmodium berghei ANKA (PbA). We found that both PKCtheta(+/+) and PKCtheta(-/-) C57BL/6J mice are susceptible to infection with PbA. However, despite a similar parasite burden, PKCtheta(+/+) mice had an earlier onset of neurological signs, characteristics of experimental cerebral malaria (ECM), resulting in an earlier death. These mice suffered from an early and pronounced splenomegaly with a concomitant increase in the total number of CD4(+) splenic T cells. In contrast, a large proportion of PbA-infected PKCtheta(-/-) mice overcame the acute phase characterized by neurological symptoms and survived longer than PKCtheta(+/+) mice. The partial resistance of PKCtheta(-/-) mice to ECM was associated with an impaired production of Th1-type cytokines, including gamma interferon and tumor necrosis factor alpha/lymphotoxin-alpha, which are known to exacerbate symptoms leading to ECM. In addition, PbA infection-induced LFA-1 expression in CD8(+) T cells was suppressed in PKCtheta-deficient T cells, suggesting a diminished ability to adhere to endothelial cells and sequester in brain microvasculature, which may explain the decrease in neurological symptoms. These data implicate PKCtheta in CD4(+) Th1(+) and CD8(+) T-cell-mediated immune responses during PbA infection that contribute to the development of ECM. |
