| First Author | Cohen S | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 385 |
| Issue | 1 | Pages | 22-7 |
| PubMed ID | 19433059 | Mgi Jnum | J:150589 |
| Mgi Id | MGI:3851048 | Doi | 10.1016/j.bbrc.2009.05.021 |
| Citation | Cohen S, et al. (2009) PKCtheta is required for hemostasis and positive regulation of thrombin-induced platelet aggregation and alpha-granule secretion. Biochem Biophys Res Commun 385(1):22-7 |
| abstractText | Platelet activation due to vascular injury is essential for hemostatic plug formation, and is mediated by agonists, such as thrombin, which trigger distinct receptor-coupled signaling pathways. Thrombin is a coagulation protease, which activates G protein-coupled protease-activated receptors (PARs) on the surface of platelets. We found that C57BL/6J and BALB/C mice that are deficient in protein kinase C theta (PKCtheta), exhibit an impaired hemostasis, and prolonged bleeding following vascular injury. In addition, murine platelets deficient in PKCtheta displayed an impaired thrombin-induced platelet activation and aggregation response. Lack of PKCtheta also resulted in impaired alpha-granule secretion, as demonstrated by the low surface expression of CD62P, in thrombin-stimulated platelets. Since PAR4 is the only mouse PAR receptor that delivers thrombin-induced activation signals in platelets, our results suggest that PKCtheta is a critical effector molecule in the PAR4-linked signaling pathways and in the regulation of normal hemostasis in mice. |
