| First Author | Ding Z | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 11 | Pages | e81299 |
| PubMed ID | 24250831 | Mgi Jnum | J:209687 |
| Mgi Id | MGI:5568314 | Doi | 10.1371/journal.pone.0081299 |
| Citation | Ding Z, et al. (2013) Complement-activating IgM enhances the humoral but not the T cell immune response in mice. PLoS One 8(11):e81299 |
| abstractText | IgM antibodies specific for a certain antigen can enhance antibody responses when administered together with this antigen, a process believed to require complement activation by IgM. However, recent data show that a knock-in mouse strain, Cmu13, which only produces IgM unable to activate complement, has normal antibody responses. Moreover, the recently discovered murine IgM Fc receptor (FcmicroR or TOSO/FAIM3) was shown to affect antibody responses. This prompted the re-investigation of whether complement activation by specific IgM is indeed required for enhancement of antibody responses and whether the mutation in Cmicro13 IgM also caused impaired binding to FcmicroR. The results show that IgM from Cmicro13 and wildtype mice bound equally well to the murine FcmicroR. In spite of this, specific Cmu13 IgM administered together with sheep red blood cells or keyhole limpet hemocyanine was a very poor enhancer of the antibody and germinal center responses as compared with wildtype IgM. Within seconds after immunization, wildtype IgM induced deposition of C3 on sheep red blood cells in the blood. IgM which efficiently enhanced the T-dependent humoral immune response had no effect on activation of specific CD4(+) T cells as measured by cell numbers, cell division, blast transformation, or expression of the activation markers LFA-1 and CD44 in vivo. These observations confirm the importance of complement for the ability of specific IgM to enhance antibody responses and suggest that there is a divergence between the regulation of T- and B-cell responses by IgM. |
