| First Author | Nishikawa H | Year | 2005 |
| Journal | J Exp Med | Volume | 201 |
| Issue | 5 | Pages | 681-6 |
| PubMed ID | 15753203 | Mgi Jnum | J:97856 |
| Mgi Id | MGI:3576533 | Doi | 10.1084/jem.20041959 |
| Citation | Nishikawa H, et al. (2005) Definition of target antigens for naturally occurring CD4+ CD25+ regulatory T cells. J Exp Med 201(5):681-6 |
| abstractText | The antigenic targets recognized by naturally occurring CD4(+) CD25(+) regulatory T cells (T reg cells) have been elusive. We have serologically defined a series of broadly expressed self-antigens derived from chemically induced mouse sarcomas by serological identification of antigens by recombinant expression cloning (SEREX). CD4(+) CD25(+) T cells from mice immunized with SEREX-defined self-antigens had strong suppressive activity on peptide-specific proliferation of CD4(+) CD25(-) T cells and CD8(+) T cells. The suppressive effect was observed without in vitro T cell stimulation. Foxp3 expression in these CD4(+) CD25(+) T cells from immunized mice was 5-10 times greater than CD4(+) CD25(+) T cells derived from naive mice. The suppressive effect required cellular contact and was blocked by anti-glucocorticoid-induced tumor necrosis factor receptor family-related gene antibody. In vitro suppressive activity essentially disappeared 8 wk after the last immunization. However, it was regained by in vitro restimulation with cognate self-antigen protein but not with control protein. We propose that SEREX-defined self-antigens such as those used in this study represent self-antigens that elicit naturally occurring CD4(+) CD25(+) T reg cells. |
