| First Author | Phee H | Year | 2010 |
| Journal | Nat Immunol | Volume | 11 |
| Issue | 6 | Pages | 503-11 |
| PubMed ID | 20431621 | Mgi Jnum | J:160613 |
| Mgi Id | MGI:4454724 | Doi | 10.1038/ni.1868 |
| Citation | Phee H, et al. (2010) Regulation of thymocyte positive selection and motility by GIT2. Nat Immunol 11(6):503-11 |
| abstractText | Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein-coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2(-/-) double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2(-/-) thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes. |
