| First Author | Zhang Y | Year | 2014 |
| Journal | Int Immunol | Volume | 26 |
| Issue | 1 | Pages | 47-57 |
| PubMed ID | 24080084 | Mgi Jnum | J:207033 |
| Mgi Id | MGI:5554324 | Doi | 10.1093/intimm/dxt042 |
| Citation | Zhang Y, et al. (2014) A role for CMTM7 in BCR expression and survival in B-1a but not B-2 cells. Int Immunol 26(1):47-57 |
| abstractText | B-1 cells are an important cell population for the production of natural antibodies and front-line host defense. Here, we show that the MARVEL-domain-containing membrane protein CMTM7 (CKLF-like MARVEL transmembrane domain-containing 7) plays a critical role in BCR expression and survival in B-1a cells. We analyzed lymphocyte development in Rag1(-)/(-) mice reconstituted with Cmtm7(flox/(+)) fetal liver cells because of the unexpected lethality of the Cmtm7(flox/(+)) heterozygotes. We found a mild reduction of serum IgM and a significantly reduced B-1a population in the peritoneal cavity of Rag1(-)/(-) mice reconstituted with Cmtm7(flox/(+)) cells compared with those reconstituted with wild-type (WT) cells. The reduction of B-1a cells in Cmtm7(flox/(+)) mice was associated with reduced BCR expression and increased spontaneous cell death in these cells. In addition, both B-1a and B-1b cells derived from Cmtm7(flox/(+)) fetal liver cells contained a lower frequency of cells capable of spontaneously differentiating into IgM-secreting plasma cells than did those derived from WT fetal liver cells. Furthermore, Cmtm7(flox/(+)) B-1a and B-1b cells responded poorly to LPS-induced proliferation. In striking contrast to the defects in B-1 cells, Cmtm7(flox/(+)) B-2 cells did not show obvious abnormalities when compared with WT B-2 cells. These results demonstrate a specific role for CMTM7 in BCR expression and survival in B-1a cells. |
