| First Author | Kashiwakura JI | Year | 2019 |
| Journal | Int Immunol | Volume | 31 |
| Issue | 5 | Pages | 349-356 |
| PubMed ID | 30726917 | Mgi Jnum | J:276903 |
| Mgi Id | MGI:6315723 | Doi | 10.1093/intimm/dxz013 |
| Citation | Kashiwakura JI, et al. (2019) STAP-2 positively regulates FcepsilonRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. Int Immunol 31(5):349-356 |
| abstractText | Basophils are an important cell type in the regulation of Th2 immune responses. Recently, we revealed that signal-transducing adaptor protein-2 (STAP-2) negatively regulates mast cell activation via FcepsilonRI. However, the role of STAP-2 in basophil maturation and activation remained unclear. In this study, we demonstrated the normal development of basophils in STAP-2-deficient (STAP-2-/-) mice. We also demonstrated in vitro normal basophil differentiation and FcepsilonRI expression in STAP-2-/- mice, suggesting that STAP-2 is dispensable for basophil maturation. Using bone marrow-derived cultured basophils (BMBs), we showed that degranulation and cytokine production of STAP-2-/- BMBs were lower than those of wild-type (WT) BMBs upon stimulation with IgE/Ag. In accordance with the reduction of degranulation and cytokine production, phosphorylation of several signal molecules such as Lyn, PLC-gamma2 and Erk was reduced in STAP-2-/- BMBs after stimulation via FcepsilonRI. Finally, it was observed that IgE-dependent chronic allergic inflammation of STAP-2-/- mice was significantly inhibited compared with WT mice. Taken together, we conclude that STAP-2 is an adaptor molecule that positively regulates FcepsilonRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. |
