| First Author | Haan KD | Year | 2023 |
| Journal | iScience | Volume | 26 |
| Issue | 3 | Pages | 106258 |
| PubMed ID | 36926650 | Mgi Jnum | J:334969 |
| Mgi Id | MGI:7446042 | Doi | 10.1016/j.isci.2023.106258 |
| Citation | Haan KD, et al. (2023) Osmotically evoked PLCdelta1-dependent translocation of DeltaN-TRPV1 channels in rat supraoptic neurons. iScience 26(3):106258 |
| abstractText | Osmoregulation is an essential homeostatic process that requires constant release of vasopressin during sustained increases in plasma osmolality. The magnocellular neurosecretory cells (MNCs) respond to increases in external osmolality through increases in the activity of DeltaN-TRPV1 channels, which leads to increased action potential firing and vasopressin release. We show that sustained exposure of acutely isolated rat and mouse MNCs to hypertonic solutions (90 min) causes a reversible translocation of DeltaN-TRPV1 channels from internal stores to the plasma membrane that depends on the activation of phospholipase C and on SNARE-dependent exocytosis. DeltaN-TRPV1 channel translocation is absent in MNCs isolated from transgenic mice lacking the PLCdelta1 isoform, suggesting that PLCdelta1 is essential for triggering this process. The translocation of DeltaN-TRPV1 channels to the cell surface could contribute to the maintenance of MNC excitability during sustained increases in osmolality. Our data therefore have important implications for the mechanisms underlying mammalian osmoregulation. |
