| First Author | Kerstein PC | Year | 2020 |
| Journal | Cell Rep | Volume | 33 |
| Issue | 7 | Pages | 108382 |
| PubMed ID | 33207201 | Mgi Jnum | J:323530 |
| Mgi Id | MGI:6714853 | Doi | 10.1016/j.celrep.2020.108382 |
| Citation | Kerstein PC, et al. (2020) Gbx2 Identifies Two Amacrine Cell Subtypes with Distinct Molecular, Morphological, and Physiological Properties. Cell Rep 33(7):108382 |
| abstractText | Our understanding of nervous system function is limited by our ability to identify and manipulate neuronal subtypes within intact circuits. We show that the Gbx2(CreERT2-IRES-EGFP) mouse line labels two amacrine cell (AC) subtypes in the mouse retina that have distinct morphological, physiological, and molecular properties. Using a combination of RNA-seq, genetic labeling, and patch clamp recordings, we show that one subtype is GABAergic that receives excitatory input from On bipolar cells. The other population is a non-GABAergic, non-glycinergic (nGnG) AC subtype that lacks the expression of standard neurotransmitter markers. Gbx2(+) nGnG ACs have smaller, asymmetric dendritic arbors that receive excitatory input from both On and Off bipolar cells. Gbx2(+) nGnG ACs also exhibit spatially restricted tracer coupling to bipolar cells (BCs) through gap junctions. This study identifies a genetic tool for investigating the two distinct AC subtypes, and it provides a model for studying synaptic communication and visual circuit function. |
