| First Author | Shen J | Year | 2006 |
| Journal | Neuroreport | Volume | 17 |
| Issue | 2 | Pages | 135-9 |
| PubMed ID | 16407759 | Mgi Jnum | J:104926 |
| Mgi Id | MGI:3613133 | Doi | 10.1097/01.wnr.0000199459.16789.75 |
| Citation | Shen J, et al. (2006) Functional expression of transient receptor potential vanilloid 4 in the mouse cochlea. Neuroreport 17(2):135-139 |
| abstractText | Transient receptor potential vanilloid 4, the Ca-permeable cation channel has been proposed as an osmosensitive and a mechanosensitive channel. We investigated functional expression of transient receptor potential vanilloid 4 in inner hair cells, outer hair cells, and spiral ganglion neurons of the mouse cochlea. Transient receptor potential vanilloid 4 mRNA and protein were expressed in inner hair cells, outer hair cells, and spiral ganglion neurons on the basis of the findings of reverse transcriptase-polymerase chain reaction, single-cell reverse transcriptase-polymerase chain reaction, and immunohistochemistry, whereas they were negative in transient receptor potential vanilloid 4 mice cochleae. Hypotonic stimulation and 4-alpha-phorbol 12,13-didecanoate, a transient receptor potential vanilloid 4 synthetic activator, increased the intracellular Ca concentrations in wild-type outer hair cells, whereas in transient receptor potential vanilloid 4 mice, outer hair cells failed to exhibit a Ca response to both stimulations. In conclusion, transient receptor potential vanilloid 4 may function as an osmosensory and a mechanosensory receptor in the cochlea. |
