| First Author | Zwijnenburg AJ | Year | 2023 |
| Journal | Immunity | Volume | 56 |
| Issue | 8 | Pages | 1955-1974.e10 |
| PubMed ID | 37490909 | Mgi Jnum | J:354093 |
| Mgi Id | MGI:7518523 | Doi | 10.1016/j.immuni.2023.06.025 |
| Citation | Zwijnenburg AJ, et al. (2023) Graded expression of the chemokine receptor CX3CR1 marks differentiation states of human and murine T cells and enables cross-species interpretation. Immunity 56(8):1955-1974.e10 |
| abstractText | T cells differentiate into functionally distinct states upon antigen encounter. These states are delineated by different cell surface markers for murine and human T cells, which hamper cross-species translation of T cell properties. We aimed to identify surface markers that reflect the graded nature of CD8(+) T cell differentiation and delineate functionally comparable states in mice and humans. CITEseq analyses revealed that graded expression of CX3CR1, encoding the chemokine receptor CX3CR1, correlated with the CD8(+) T cell differentiation gradient. CX3CR1 expression distinguished human and murine CD8(+) and CD4(+) T cell states, as defined by migratory and functional properties. Graded CX3CR1 expression, refined with CD62L, accurately captured the high-dimensional T cell differentiation continuum. Furthermore, the CX3CR1 expression gradient delineated states with comparable properties in humans and mice in steady state and on longitudinally tracked virus-specific CD8(+) T cells in both species. Thus, graded CX3CR1 expression provides a strategy to translate the behavior of distinct T cell differentiation states across species. |
