| First Author | Chinnery HR | Year | 2012 |
| Journal | Neurobiol Aging | Volume | 33 |
| Issue | 8 | Pages | 1769-76 |
| PubMed ID | 21570740 | Mgi Jnum | J:188207 |
| Mgi Id | MGI:5439694 | Doi | 10.1016/j.neurobiolaging.2011.03.010 |
| Citation | Chinnery HR, et al. (2012) Accumulation of murine subretinal macrophages: effects of age, pigmentation and CX3CR1. Neurobiol Aging 33(8):1769-76 |
| abstractText | Macrophages or activated microglia in the subretinal space are considered a hallmark of some retinal pathologies. We investigated the effects of age, pigmentation and CX(3)CR1 deficiency on the accumulation of macrophages/activated microglia in the outer retina of young and old Cx(3)cr1(gfp/gfp) (CX(3)CR1-deficient) or Cx(3)cr1(gfp/+) mice on either a pigmented (C57BL/6) or albino (BALB/c) background. Quantitative analysis of immunostained retinal-choroidal whole mounts revealed an increase in subretinal macrophage (SRMPhi) numbers in young Cx(3)cr1(gfp/gfp) mice compared with Cx(3)cr1(gfp/+) mice, however the increase was more marked in albino Cx(3)cr1(gfp/gfp) mice. In aged mice, large numbers of SRMPhi/activated microglia replete with autofluorescent debris were noted in both old pigmented Cx(3)cr1(gfp/gfp) and Cx(3)cr1(gfp/+) mice proving this accumulation was not CX(3)CR1-dependent. While CX(3)CR1 deficiency leads to an early onset of SRMPhi accumulation, our data reveal that this change occurs in both aged Cx(3)cr1(gfp/+) and Cx(3)cr1(gfp/gfp) pigmented mice in the absence of marked retinal degeneration and is likely a normal response to aging. |
