| First Author | Jin BH | Year | 2023 |
| Journal | Exp Mol Med | Volume | 55 |
| Issue | 10 | Pages | 2190-2204 |
| PubMed ID | 37779150 | Mgi Jnum | J:342150 |
| Mgi Id | MGI:7545860 | Doi | 10.1038/s12276-023-01089-8 |
| Citation | Jin BH, et al. (2023) Optimization of the optical transparency of bones by PACT-based passive tissue clearing. Exp Mol Med 55(10):2190-2204 |
| abstractText | Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and biological circuits. Nonetheless, the issues that remain in maintaining structural integrity and preventing tissue expansion/shrinkage with rapid clearing still inhibit the wide application of these techniques in hard bone tissues, such as femurs and tibias. Here, we present an optimized PACT-based bone-clearing method, Bone-mPACT+, that protects biological structures. Bone-mPACT+ and four different decalcifying procedures were tested for their ability to improve bone tissue clearing efficiency without sacrificing optical transparency; they rendered nearly all types of bone tissues transparent. Both mouse and rat bones were nearly transparent after the clearing process. We also present a further modification, the Bone-mPACT+ Advance protocol, which is specifically optimized for processing the largest and hardest rat bones for easy clearing and imaging using established tissue clearing methods. |
