| First Author | Cheng Y | Year | 2015 |
| Journal | Endocrinology | Volume | 156 |
| Issue | 7 | Pages | 2724-31 |
| PubMed ID | 25885930 | Mgi Jnum | J:224637 |
| Mgi Id | MGI:5688448 | Doi | 10.1210/en.2015-1104 |
| Citation | Cheng Y, et al. (2015) Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic beta-Cell-Specific Gene Overexpression and Knockout. Endocrinology 156(7):2724-31 |
| abstractText | The technologies for pancreatic beta-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to beta-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate beta-cell researches. |
