| First Author | Perera SN | Year | 2020 |
| Journal | Glia | Volume | 68 |
| Issue | 12 | Pages | 2550-2584 |
| PubMed ID | 32857879 | Mgi Jnum | J:332131 |
| Mgi Id | MGI:7408609 | Doi | 10.1002/glia.23870 |
| Citation | Perera SN, et al. (2020) Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia 68(12):2550-2584 |
| abstractText | Olfactory ensheathing cells (OECs) are neural crest-derived glia that ensheath bundles of olfactory axons from their peripheral origins in the olfactory epithelium to their central targets in the olfactory bulb. We took an unbiased laser microdissection and differential RNA-seq approach, validated by in situ hybridization, to identify candidate molecular mechanisms underlying mouse OEC development and differences with the neural crest-derived Schwann cells developing on other peripheral nerves. We identified 25 novel markers for developing OECs in the olfactory mucosa and/or the olfactory nerve layer surrounding the olfactory bulb, of which 15 were OEC-specific (that is, not expressed by Schwann cells). One pan-OEC-specific gene, Ptprz1, encodes a receptor-like tyrosine phosphatase that blocks oligodendrocyte differentiation. Mutant analysis suggests Ptprz1 may also act as a brake on OEC differentiation, and that its loss disrupts olfactory axon targeting. Overall, our results provide new insights into OEC development and the diversification of neural crest-derived glia. |
