| First Author | Zhang Y | Year | 2009 |
| Journal | FEBS Lett | Volume | 583 |
| Issue | 18 | Pages | 3101-6 |
| PubMed ID | 19716369 | Mgi Jnum | J:153076 |
| Mgi Id | MGI:4360834 | Doi | 10.1016/j.febslet.2009.08.027 |
| Citation | Zhang Y, et al. (2009) Epinephrine-induced hyperpolarization of pancreatic islet cells is sensitive to PI3K-PDK1 signaling. FEBS Lett 583(18):3101-6 |
| abstractText | Epinephrine inhibits insulin release by activation of K(+) channels and subsequent hyperpolarization of pancreatic beta cells. The present study explored whether epinephrine-induced hyperpolarization is modified by phosphatidylinositol 3-kinase (PI3K) and phosphatidylinositide-dependent kinase PDK1. Perforated patch-clamp was performed in islet cells isolated from PDK1 hypomorphic mice (pdk1(fl/fl)), expressing only 20% of PDK1, and in their wild-type littermates. At 16.8mM glucose, the cell membrane was hyperpolarized by epinephrine (1 microM), an effect significantly blunted in pdk1(fl/fl) and abrogated in wild-type cells by inhibition of PI3K with wortmannin (100 nM) or LY294002 (10 microM). The hyperpolarizing effect of epinephrine in pancreatic islet cells is thus sensitive to PI3K and PDK1. |
