| Primary Identifier | MGI:2677700 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ccne2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 12.3 kb region of genomic sequence starting with the 5' UTR and including all coding exons as well as part of the 3' UTR were replaced with a floxed neomycin resistance gene. Transient transfection with a cre expressing plasmid then eliminated the neomycin gene. Absence of mRNA transcripts in mice derived from these ES cells was demonstrated by reverse transcription and PCR. |
