| First Author | Shan B | Year | 2022 |
| Journal | Cell Metab | Volume | 34 |
| Issue | 5 | Pages | 783-799.e7 |
| PubMed ID | 35447091 | Mgi Jnum | J:353663 |
| Mgi Id | MGI:7286146 | Doi | 10.1016/j.cmet.2022.03.012 |
| Citation | Shan B, et al. (2022) Multilayered omics reveal sex- and depot-dependent adipose progenitor cell heterogeneity. Cell Metab 34(5):783-799.e7 |
| abstractText | Single-cell RNA sequencing (scRNA-seq) has revealed that adult white adipose tissue (WAT) harbors functionally diverse subpopulations of mesenchymal stromal cells that differentially impact tissue plasticity. To date, the molecular basis of this cellular heterogeneity has not been fully defined. Here, we describe a multilayered omics approach to dissect adipose progenitor cell heterogeneity in three dimensions: progenitor subpopulation, sex, and anatomical localization. We applied state-of-the-art mass spectrometry methods to quantify 4,870 proteins in eight different stromal cell populations from perigonadal and inguinal WAT of male and female mice and acquired transcript expression levels of 15,477 genes using RNA-seq. Our data unveil molecular signatures defining sex differences in preadipocyte differentiation and identify regulatory pathways that functionally distinguish adipose progenitor subpopulations. This multilayered omics analysis, freely accessible at http://preadprofiler.net/, provides unprecedented insights into adipose stromal cell heterogeneity and highlights the benefit of complementary proteomics to support findings from scRNA-seq studies. |
