| First Author | Pardo-Saganta A | Year | 2015 |
| Journal | Cell Stem Cell | Volume | 16 |
| Issue | 2 | Pages | 184-97 |
| PubMed ID | 25658372 | Mgi Jnum | J:224011 |
| Mgi Id | MGI:5661098 | Doi | 10.1016/j.stem.2015.01.002 |
| Citation | Pardo-Saganta A, et al. (2015) Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations. Cell Stem Cell 16(2):184-97 |
| abstractText | Following injury, stem cells restore normal tissue architecture by producing the proper number and proportions of differentiated cells. Current models of airway epithelial regeneration propose that distinct cytokeratin 8-expressing progenitor cells, arising from p63(+) basal stem cells, subsequently differentiate into secretory and ciliated cell lineages. We now show that immediately following injury, discrete subpopulations of p63(+) airway basal stem/progenitor cells themselves express Notch pathway components associated with either secretory or ciliated cell fate commitment. One basal cell population displays intracellular Notch2 activation and directly generates secretory cells; the other expresses c-myb and directly yields ciliated cells. Furthermore, disrupting Notch ligand activity within the basal cell population at large disrupts the normal pattern of lineage segregation. These non-cell-autonomous effects demonstrate that effective airway epithelial regeneration requires intercellular communication within the broader basal stem/progenitor cell population. These findings have broad implications for understanding epithelial regeneration and stem cell heterogeneity. |
