| First Author | Lüönd F | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 2 | Pages | 101438 |
| PubMed ID | 35707685 | Mgi Jnum | J:349089 |
| Mgi Id | MGI:7646089 | Doi | 10.1016/j.xpro.2022.101438 |
| Citation | Luond F, et al. (2022) Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer. STAR Protoc 3(2):101438 |
| abstractText | The various stages of epithelial-mesenchymal transition (EMT) generate phenotypically heterogeneous populations of cells. Here, we detail a dual recombinase lineage tracing system using a transgenic mouse model of metastatic breast cancer to trace and characterize breast cancer cells at different EMT stages. We describe analytical steps to label cancer cells at an early partial or a late full EMT state, followed by tracking their behavior in tumor slice cultures. We then characterize their transcriptome by five-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Luond et al. (2021). |
