| First Author | Cui J | Year | 2012 |
| Journal | Neuroscience | Volume | 224 |
| Pages | 63-9 | PubMed ID | 22909426 |
| Mgi Jnum | J:192487 | Mgi Id | MGI:5465243 |
| Doi | 10.1016/j.neuroscience.2012.08.017 | Citation | Cui J, et al. (2012) Expression of CaV3.2 T-type Ca(2)(+) channels in a subpopulation of retinal type-3 cone bipolar cells. Neuroscience 224:63-9 |
| abstractText | Retinal bipolar cells and ganglion cells are known to possess voltage-gated T-type Ca(2+) channels. Previous electrophysiological recording studies suggested that there is differential expression of different T-type Ca(2+) channel alpha1 subunits among bipolar cells. The detailed expression patterns of the individual T-type Ca(2+) channel subunits in the retina, however, remain unknown. In this study, we examined the expression of the Ca(V)3.2 Ca(2+) channel alpha1 subunit in the mouse retina using immunohistochemical analysis and patch-clamp recordings together with a Ca(V)3.2 knock out (KO) mouse line. The specificity of a Ca(V)3.2 Ca(2+) channel antibody was first confirmed in recombinant T-type Ca(2+) channels expressed in human embryonic kidney (HEK) cells and in Ca(V)3.2 KO mice. Our immunohistochemical analysis indicates that the Ca(V)3.2 antibody labels a subgroup of type-3 cone bipolar cells (CBCs), the PKAbetaII-immunopositive type-3 CBCs. The labeling was observed throughout the cell including dendrites and axon terminals. Our patch-clamp recording results further demonstrate that Ca(V)3.2 Ca(2+) channels contribute to the T-type Ca(2+) current in a subpopulation of type-3 CBCs. The findings of this study provide new insights into understanding the functional roles of T-type Ca(2+) channels in retinal processing. |
