| First Author | Shimada R | Year | 2021 |
| Journal | iScience | Volume | 24 |
| Issue | 8 | Pages | 102890 |
| PubMed ID | 34401671 | Mgi Jnum | J:309182 |
| Mgi Id | MGI:6756275 | Doi | 10.1016/j.isci.2021.102890 |
| Citation | Shimada R, et al. (2021) NANOS2 suppresses the cell cycle by repressing mTORC1 activators in embryonic male germ cells. iScience 24(8):102890 |
| abstractText | During murine germ cell development, male germ cells enter the mitotically arrested G0 stage, which is an initial step of sexually dimorphic differentiation. The male-specific RNA-binding protein NANOS2 has a key role in suppressing the cell cycle in germ cells. However, the detailed mechanism of how NANOS2 regulates the cell cycle remains unclear. Using single-cell RNA sequencing (scRNA-seq), we extracted the cell cycle state of each germ cell in wild-type and Nanos2-KO testes and revealed that Nanos2 expression starts in mitotic cells and induces mitotic arrest. We identified Rheb, a regulator of mTORC1, and Ptma as possible targets of NANOS2. We propose that repression of the cell cycle is a primary function of NANOS2 and that it is mediated via the suppression of mTORC1 activity through the repression of Rheb in a post-transcriptional manner. |
