| First Author | Fields B | Year | 2019 |
| Journal | Sci Rep | Volume | 9 |
| Issue | 1 | Pages | 19303 |
| PubMed ID | 31848396 | Mgi Jnum | J:287117 |
| Mgi Id | MGI:6406215 | Doi | 10.1038/s41598-019-55844-x |
| Citation | Fields B, et al. (2019) The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis. Sci Rep 9(1):19303 |
| abstractText | Laminin-gamma1 is required for early embryonic development; however, the need for laminin-gamma1 synthesis in adulthood is unknown. A global and inducible mouse model of laminin-gamma1 deficiency was generated to address this question. Genetic ablation of the Lamc1 gene in adult mice was rapidly lethal. Despite global Lamc1 gene deletion in tamoxifen-induced mutant mice, there was minimal change in total cardiac, pulmonary, hepatic or renal laminin protein. In contrast, laminin-gamma1 was significantly depleted in the small intestines, which showed crypt hyperplasia and dissociation of villous epithelium from adjacent mesenchyme. We conclude that the physiologic requirement for laminin-gamma1 synthesis in adult mice is dependent on a tissue-specific basal rate of laminin-gamma1 turnover that results in rapid depletion of laminin-gamma1 in the intestine. |
