| First Author | Hansen AH | Year | 2024 |
| Journal | STAR Protoc | Volume | 5 |
| Issue | 1 | Pages | 102795 |
| PubMed ID | 38165800 | Mgi Jnum | J:347035 |
| Mgi Id | MGI:7618880 | Doi | 10.1016/j.xpro.2023.102795 |
| Citation | Hansen AH, et al. (2024) Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers. STAR Protoc 5(1):102795 |
| abstractText | Mosaic analysis with double markers (MADM) technology enables the sparse labeling of genetically defined neurons. We present a protocol for time-lapse imaging of cortical projection neuron migration in mice using MADM. We describe steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled brain tissue. While this protocol is compatible with other single-cell labeling methods, the MADM approach provides a genetic platform for the functional assessment of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous effects. For complete details on the use and execution of this protocol, please refer to Hansen et al. (2022),(1) Contreras et al. (2021),(2) and Amberg and Hippenmeyer (2021).(3). |
