| Primary Identifier | MGI:2686951 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hcn1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of the pore-S6 domain containing exon. At the same time, a floxed neomycin cassette was introduced downstream. Cells in which transient Cre recombinase expression of properly targeted ES cells resulted in excision of both the neomycin gene and the pore-S6 exon were selected. Both mRNA and protein were shown to be absent in the brains of mice homozygous for this allele. |
