| First Author | Moon SJ | Year | 2020 |
| Journal | J Cell Mol Med | Volume | 24 |
| Issue | 15 | Pages | 8814-8825 |
| PubMed ID | 32570293 | Mgi Jnum | J:298214 |
| Mgi Id | MGI:6477447 | Doi | 10.1111/jcmm.15519 |
| Citation | Moon SJ, et al. (2020) Ablation of Gadd45beta ameliorates the inflammation and renal fibrosis caused by unilateral ureteral obstruction. J Cell Mol Med 24(15):8814-8825 |
| abstractText | The growth arrest and DNA damage-inducible beta (Gadd45beta) protein have been associated with various cellular functions, but its role in progressive renal disease is currently unknown. Here, we examined the effect of Gadd45beta deletion on cell proliferation and apoptosis, inflammation, and renal fibrosis in an early chronic kidney disease (CKD) mouse model following unilateral ureteral obstruction (UUO). Wild-type (WT) and Gadd45beta-knockout (KO) mice underwent either a sham operation or UUO and the kidneys were sampled eight days later. A histological assay revealed that ablation of Gadd45beta ameliorated UUO-induced renal injury. Cell proliferation was higher in Gadd45beta KO mouse kidneys, but apoptosis was similar in both genotypes after UUO. Expression of pro-inflammatory cytokines after UUO was down-regulated in the kidneys from Gadd45beta KO mice, whereas UUO-mediated immune cell infiltration remained unchanged. The expression of pro-inflammatory cytokines in response to LPS stimulation decreased in bone marrow-derived macrophages from Gadd45beta KO mice compared with that in WT mice. Importantly, UUO-induced renal fibrosis was ameliorated in Gadd45beta KO mice unlike in WT mice. Gadd45beta was involved in TGF-beta signalling pathway regulation in kidney fibroblasts. Our findings demonstrate that Gadd45beta plays a crucial role in renal injury and may be a therapeutic target for the treatment of CKD. |
