| Primary Identifier | MGI:2683707 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | St14 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A genomic fragment encompassing 350 bp of the putative intron 1 and the majority of the putative exon 2 was replaced with an hprt cassette inserted by homologous recombination. The deleted region encoded 19 of the 24 residues of the signal anchor required for membrane translocation. RT-PCR analysis identified mutant transcript in which the putative exon 1 spliced to the putative exon 3. This aberrant splice event results in a frameshift and ultimately a premature stop codon. |
