Primary Identifier | MGI:2687297 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Pax3 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 3' portion of exon 2 was replaced with a cassette containing IRES-lacZ followed by a polyadenylation signal. The targeting vector contained a floxed puromycin resistance gene, however this was excised via cre mediated recombination, leaving only a single loxP site following the lacZ cassette. The expression pattern of lacZ was shown to be similar to that of the endogenous gene. |