| Primary Identifier | MGI:2684310 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mbd4 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+) or W9.5 (129S1/Sv-p+ Tyr+ Kitl+). |
| molecularNote | A genomic fragment encompassing exons 2, 3, 4, and 5 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded portions of the 5-methylcytosine binding and catalytic domains. Transcript was undetected by RT-PCR analysis of homozygous MEFs. |
