| Primary Identifier | MGI:2684311 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mbd4 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+) or W9.5 (129S1/Sv-p+ Tyr+ Kitl+). |
| molecularNote | A genomic fragment encompassing exons 1, 2, and 3 of Mbd4 and exon 1 of Ift122 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the entire 5-methylcytosine binding domain and a portion of the catalytic domain. RT-PCR confirmed the absence of expression of both genes in homozygous MEFs. |
