| First Author | Conrad E | Year | 2016 |
| Journal | Am J Physiol Endocrinol Metab | Volume | 310 |
| Issue | 1 | Pages | E91-E102 |
| PubMed ID | 26554594 | Mgi Jnum | J:235909 |
| Mgi Id | MGI:5803934 | Doi | 10.1152/ajpendo.00285.2015 |
| Citation | Conrad E, et al. (2016) The MAFB transcription factor impacts islet alpha-cell function in rodents and represents a unique signature of primate islet beta-cells. Am J Physiol Endocrinol Metab 310(1):E91-E102 |
| abstractText | Analysis of MafB(-/-) mice has suggested that the MAFB transcription factor was essential to islet alpha- and beta-cell formation during development, although the postnatal physiological impact could not be studied here because these mutants died due to problems in neural development. Pancreas-wide mutant mice were generated to compare the postnatal significance of MafB (MafB(Deltapanc)) and MafA/B (MafAB(Deltapanc)) with deficiencies associated with the related beta-cell-enriched MafA mutant (MafA(Deltapanc)). Insulin(+) cell production and beta-cell activity were merely delayed in MafB(Deltapanc) islets until MafA was comprehensively expressed in this cell population. We propose that MafA compensates for the absence of MafB in MafB(Deltapanc) mice, which is supported by the death of MafAB(Deltapanc) mice soon after birth from hyperglycemia. However, glucose-induced glucagon secretion was compromised in adult MafB(Deltapanc) islet alpha-cells. Based upon these results, we conclude that MafB is only essential to islet alpha-cell activity and not beta-cell. Interestingly, a notable difference between mice and humans is that MAFB is coexpressed with MAFA in adult human islet beta-cells. Here, we show that nonhuman primate (NHP) islet alpha- and beta-cells also produce MAFB, implying that MAFB represents a unique signature and likely important regulator of the primate islet beta-cell. |
