| First Author | Wolf I | Year | 1998 |
| Journal | J Biol Chem | Volume | 273 |
| Issue | 44 | Pages | 28831-6 |
| PubMed ID | 9786883 | Mgi Jnum | J:50595 |
| Mgi Id | MGI:1306985 | Doi | 10.1074/jbc.273.44.28831 |
| Citation | Wolf I, et al. (1998) Downstream activation of a TATA-less promoter by Oct-2, Bob1, and NF- kappaB directs expression of the homing receptor BLR1 to mature B cells. J Biol Chem 273(44):28831-6 |
| abstractText | The chemokine receptor, BLR1, is a major regulator of the microenvironmental homing of B cells in lymphoid organs. In vitro studies identify three essential elements of the TATA-less blr1 core promoter that confer cell type- and differentiation-specific expression in the B cells of both humans and mice, a functional promoter region (- 36 with respect to the transcription start site), a NF-kappaB motif (+44), and a noncanonical octamer motif (+157). The importance of these sites was confirmed by in vivo studies in gene-targeted mice deficient of either Oct-2, Bob1, or both NF-kappaB subunits p50 and p52. In all of these animals, the expression of BLR1 was reduced or absent. In mice deficient only of p52/NF-kappaB, BLR1 expression was unaffected. Thus our data demonstrate that BLR1 is a target gene for Oct-2, Bob1, and members of the NF-kappaB/Rel family and provides a link to the impaired B cell functions in mice deficient for these factors. |
